Genomic analyses reveal poaching hotspots and illegal trade in pangolins from Africa to Asia.

The white-bellied pangolin (Phataginus tricuspis) is the world's most trafficked mammal and is at risk of extinction. Reducing the illegal wildlife trade requires an understanding of its origins. Using a genomic approach for tracing confiscations and analyzing 111 samples collected from known geographic localities in Africa and 643 seized scales from Asia between 2012 and 2018, we found that poaching pressures shifted over time from West to Central Africa. Recently, Cameroon's southern border has emerged as a site of intense poaching. Using data from seizures representing nearly 1 million African pangolins, we identified Nigeria as one important hub for trafficking, where scales are amassed and transshipped to markets in Asia. This origin-to-destination approach offers new opportunities to disrupt the illegal wildlife trade and to guide anti-trafficking measures.

Home and hub: pet trade and traditional medicine impact reptile populations in source locations and destinations.

The pet trade and Traditional Chinese Medicine (TCM) consumption are major drivers of global biodiversity loss. Tokay geckos (Gekko gecko) are among the most traded reptile species worldwide. In Hong Kong, pet and TCM markets sell tokay geckos while wild populations also persist. To clarify connections between trade sources and destinations, we compared genetics and stable isotopes of wild tokays in local and non-local populations to dried individuals from TCM markets across Hong Kong. We found that TCM tokays are likely not of local origin. Most wild tokays were related to individuals in South China, indicating a probable natural origin. However, two populations contained individuals more similar to distant populations, indicating pet trade origins. Our results highlight the complexity of wildlife trade impacts within trade hubs. Such trade dynamics complicate local legal regulation when endangered species are protected, but the same species might also be non-native and possibly damaging to the environment.

Discussion of wildlife trade before and during the COVID-19 pandemic in professional opinion pieces and scientific articles.

Wildlife trade is a multi-billion-dollar sector that impacts a wide range of species, and thus is of significant research and conservation interest. Wildlife trade has also become a prominent topic in the public-facing media, where coverage has intensified following the outbreak of the global COVID-19 pandemic due to the potential connection between wildlife trade and the origin of the SARS Cov2 virus. Given the importance of the media in shaping public understanding and discourse of complex topics such as wildlife trade, this could impact the implementation of and public support for policy decisions. In this study, we followed a standardised protocol to extract wildlife trade-related discussion from 285 professional opinion pieces (NGO reports or articles in conservation-themed forums) and 107 scientific articles published in two time periods: "pre-COVID" (June 1-December 31, 2019) and "during-COVID" (January 1-May 31, 2020). We compared opinion pieces and scientific articles across the two time periods and to each other to investigate potential differences in the presentation of wildlife trade and associated speakers. We found a shift in the way that wildlife trade was discussed in professional opinion pieces between the periods, in that the discussion became less specific in terms of defining the legality and purpose of trade, and the animal groups involved in the "during-COVID" period. The generalised framing of wildlife trade in our dataset also coincided with an increased discussion of highly generalised management strategies, such as blanket bans on wildlife trade. We also found that publications included more quotes from researchers in the "during-COVID" period. In both professional opinion pieces and scientific articles, we found that quotations or research were often from speakers whose affiliation region was different to the geographic range of the trade they were speaking about. This highlights the importance of incorporating local knowledge and considering the diversity of speakers and interviewees in both research and the public-facing media about the wildlife trade.

Low pangolin consumption in Hong Kong pre- and post- the COVID-19 outbreak: Conservation and health concerns both contribute to people's attitudes.

Pangolins have recently received significant media attention globally as the trade for their scales and meat is driving many species closer to extinction. As a result of this, there have been increased legal regulations placed on pangolin trade in recent years. The suggestion that pangolins may have been involved in the transmission of COVID-19 further brought the issues of pangolin consumption to the fore in 2020. However, we have little understanding of the attitudes of the general public towards pangolin consumption pre- or post the outbreak of COVID-19. We conducted surveys in Hong Kong, a critical transit hub in the trafficking routes for pangolins, in 2015 (n = 1037) and 2020 (n = 1028) to determine general attitudes towards pangolin consumption in the city, and whether these attitudes changed since the onset of COVID-19. We found low reported rates of pangolin consumption (< 1% of respondents) in both surveys, and most of the respondents who professed to eating pangolins were aged above 50. Perceptions of how trends in pangolin consumption are changing were consistent between 2015 and 2020, with 55% of the public in 2015 and 57% in 2020 believing that consumption has declined over time. In 2020, respondents cited conservation (endangered status of pangolins) and health concerns (risk of disease transmission) as the two primary reasons (> 50%) for declining attitudes toward consumption. Overall, COVID-19 does not, specifically, appear to be associated with changed perceptions of pangolin consumption in Hong Kong: > 75% of respondents stated that there is no relationship between pangolins and COVID-19, or were unsure about any such connection. Only 1% mentioned an awareness of the illegality of pangolin consumption as a reason for not consuming them. As such, our results challenge simple narratives regarding the impact of COVID-19 on pangolin consumption. We suggest that future demand reduction efforts could emphasize the conservation impact and health risks of consuming pangolins, and specifically focus on the older generations. As pangolins continue to be trafficked and threatened with extinction, further research into the perceptions and attitudes of consumers of these products is needed to inform targeted and effective interventions.

The relationships between coping styles and food intake in shiftworking nurses and midwives: a pilot study.

Shiftworkers are more likely to suffer from gastrointestinal disease and Type 2 Diabetes than the general population, likely due to their altered dietary intakes. Previous research has suggested that coping strategies and health behaviours may be linked, however, questions remain regarding these relationships in shiftworking populations. The Standard Shiftwork Index and Food Frequency Questionnaire were completed by nurses/midwives working forward rotating shifts (N=27, female=24, age=38.4 ± 13.1 y). Greater engaged coping strategy usage was associated with lower total energy, fat, carbohydrate and sugar intake (ρs>-0.1). Greater disengaged coping strategy usage was associated with greater intake of these nutrients (ρs>0.1). Results suggest that engaged coping strategies may contribute to healthier dietary choices. A greater focus on coping styles, particularly during nursing education, may improve shiftworkers' health.

Multiple constraints on urban bird communication: both abiotic and biotic noise shape songs in cities.

Ambient noise can cause birds to adjust their songs to avoid masking. Most studies investigate responses to a single noise source (e.g., low-frequency traffic noise, or high-frequency insect noise). Here, we investigated the effects of both anthropogenic and insect noise on vocalizations of four common bird species in Hong Kong. Common Tailorbirds (Orthotomus sutorius) and Eurasian Tree Sparrows (Passer montanus) both sang at a higher frequency in urban areas compared to peri-urban areas. Red-whiskered Bulbuls (Pycnonotus jocosus) in urban areas shifted the only first note of their song upwards. Swinhoe's White-eye (Zosterops simplex) vocalization changes were correlated with noise level, but did not differ between the peri-urban and urban populations. Insect noise caused the Eurasian Tree Sparrow to reduce both maximum, peak frequency, and overall bandwidth of vocalizations. Insect noise also led to a reduction in maximum frequency in Red-whiskered bulbuls. The presence of both urban noise and insect noise affected the sound of the Common Tailorbirds and Eurasian Tree Sparrows; in urban areas, they no longer increased their minimum song frequency when insect sounds were also present. These results highlight the complexity of the soundscape in urban areas. The presence of both high- and low-frequency ambient noise may make it difficult for urban birds to avoid signal masking while still maintaining their fitness in noisy cities.

International socioeconomic inequality drives trade patterns in the global wildlife market.

The wildlife trade is a major cause of species loss and a pathway for disease transmission. Socioeconomic drivers of the wildlife trade are influential at the local scale yet rarely accounted for in multinational agreements aimed at curtailing international trade in threatened species. In recent decades (1998-2018), approximately 421,000,000 threatened (i.e., CITES-listed) wild animals were traded between 226 nations/territories. The global trade network was more highly connected under conditions of greater international wealth inequality, when rich importers may have a larger economic advantage over poorer exporting nations/territories. Bilateral trade was driven primarily by socioeconomic factors at the supply end, with wealthier exporters likely to supply more animals to the global market. Our findings suggest that international policies for reducing the global wildlife trade should address inequalities between signatory states, possibly using incentive/compensation-driven programs modeled after other transnational environmental initiatives (e.g., REDD+).

Mercury exposure in sedentary and migratory Charadrius plovers distributed widely across China.

Mercury pollution is a global problem and of particular concern in high emissions areas, such as China. We studied the migratory Kentish Plover, Charadrius alexandrinus, which breeds in coastal northern/central China and the inland Qinghai Lake, and the White-faced Plover C. dealbatus, a year-round resident of coastal southern China. We measured total mercury (THg) concentrations in feather and blood samples of breeding females. We expected low levels at the remote Qinghai Lake, but we found instead that feather THg concentrations were highest there (3.89 ± 1.53 [SD] µg/g DW [n = 34]; compared to 1.29 ± 0.61 µg/g of Kentish Plover elsewhere [n = 35] and 2.08 ± 1.45 µg/g for White-faced Plover [n = 56]), a result is consistent over 2 years. When including only coastal populations in the analysis, there were no differences in THg concentrations between the two species, although White-faced Plover had more variation. Feather THg concentrations for the coastal populations are similar to other studies on plovers and sandpipers globally, with most birds under the threshold of adverse effects (3 µg/g, an estimate that itself may be too low). Nevertheless, the Qinghai Kentish Plover population has mean feather concentrations above this threshold, indicating high exposure during the nonbreeding season, and some individuals have extreme values (e.g., a bird with a blood level of 7.63 µg/g DW from Zhanjiang, south China), so further research and monitoring are needed.

Evidence for non-selective ingestion of microplastic in demersal fish.

Microplastics have been observed in >100 species of fish, with considerable variability in levels of contamination in different species and different geographic locations. Here, we investigated the incidence of microplastic in five species of demersal fish (four wild-caught species and one from a mariculture business) in Hong Kong. We observed that 54% of fish stomachs contained microplastic (hard fragments and fibres) with no significant difference in the abundance of microplastic ingested between the species, between wild and commercial fish farms, or between locations. In addition, we observed no difference between the type of microplastics (shape or composition) ingested by fish. However, we did observe spatial variation, with fish closest to the Pearl River having higher frequency of occurrence of microplastics which reinforce the evidence that fish collected close to urban area are more likely to ingest microplastics.

Genetic, phenotypic and ecological differentiation suggests incipient speciation in two Charadrius plovers along the Chinese coast.

BACKGROUND: Speciation with gene flow is an alternative to the nascence of new taxa in strict allopatric separation. Indeed, many taxa have parapatric distributions at present. It is often unclear if these are secondary contacts, e.g. caused by past glaciation cycles or the manifestation of speciation with gene flow, which hampers our understanding of how different forces drive diversification. Here we studied genetic, phenotypic and ecological aspects of divergence in a pair of incipient shorebird species, the Kentish (Charadrius alexandrinus) and the White-faced Plovers (C. dealbatus), shorebirds with parapatric breeding ranges along the Chinese coast. We assessed divergence based on molecular markers with different modes of inheritance and quantified phenotypic and ecological divergence in aspects of morphometric, dietary and climatic niches. RESULTS: Our integrative analyses revealed small to moderate levels of genetic and phenotypic distinctiveness with symmetric gene flow across the contact area at the Chinese coast. The two species diverged approximately half a million years ago in dynamic isolation with secondary contact occurring due to cycling sea level changes between the Eastern and Southern China Sea in the mid-late Pleistocene. We found evidence of character displacement and ecological niche differentiation between the two species, invoking the role of selection in facilitating divergence despite gene flow. CONCLUSION: These findings imply that ecology can indeed counter gene flow through divergent selection and thus contributes to incipient speciation in these plovers. Furthermore, our study highlights the importance of using integrative datasets to reveal the evolutionary history and assist the inference of mechanisms of speciation.

Integrating Proximal and Horizon Threats to Biodiversity for Conservation.

Global conservation promotes solutions to different dimensions of threat and response: land-use change, climate change, pollution, and so forth. Countering each threat has its band of proponents who advocate for their cause as paramount, increasingly, given limited resources, by downplaying the relative importance of others. Not only does this encourage a compartmentalised view of the world, which is ecologically unsound, it allows politicians and others to cherry-pick responses in light of political expediency or local demands. We should instead aim to achieve win-win conservation strategies that address multiple threats to diversity acting at different timescales, as well as 'horizon threats', which occur at large scales and may be the most challenging conservation issues to address in both the present and the future.

Whale Shark Tourism: Impacts on Coral Reefs in the Philippines.

Reef-based tourism has been developing rapidly in recent decades yet its impacts on reef ecosystems are often overlooked. In Tan-awan, Oslob, Philippines, whale sharks are attracted to the shallow reefs where they are provisioned up to 50 tons y-1 of feed and this phenomenon in turn attracts >300,000 y-1 visitors. Given the intensive provisioning and concentrating tourism activities, we hypothesized that the whale shark tourism-impacted site (IS) will have greater impacts on reef degradation and higher anthropogenic nitrogen pollution level compared to its reference site (RS). Ecological surveys revealed that relative to the RS, the IS had 36% higher relative abundance of Pocillopora and Porites coral over other genera, >2.5-fold lower coral density, and 20% higher macroalgal cover, which we concluded are signs of reef degradation. Also, we conducted stable nitrogen isotope analysis on gorgonian skeletons to trace nitrogen sources at both sites through time. Although an average 1‰ isotope enrichment found in the IS relative to the RS could indicate anthropogenic nitrogen inputs in the IS, this enrichment was consistent over time and existed before the tourism developed. Despite that, we cautioned against the imminent threat of local eutrophication caused by the continued inputs of nitrogen derived from provisioning and tourism activities. In summary, this study provided the first documentation of the impacts of provisioned whale shark tourism on the local reefs in Tan-awan and established an ecological baseline for future comparisons. Such assessments can offer important information on reef health, coastal development, and tourism management.

The Environmental Exposures and Inner- and Intercity Traffic Flows of the Metro System May Contribute to the Skin Microbiome and Resistome.

The skin functions as the primary interface between the human body and the external environment. To understand how the microbiome varies within urban mass transit and influences the skin microbiota, we profiled the human palm microbiome after contact with handrails within the Hong Kong Mass Transit Railway (MTR) system. Intraday sampling time was identified as the primary determinant of the variation and recurrence of the community composition, whereas human-associated species and clinically important antibiotic resistance genes (ARGs) were captured as p.m. signatures. Line-specific signatures were notably correlated with line-specific environmental exposures and city characteristics. The sole cross-border line appeared as an outlier in most analyses and showed high relative abundance and a significant intraday increment of clinically important ARGs (24.1%), suggesting potential cross-border ARG transmission, especially for tetracycline and vancomycin resistance. Our study provides an important reference for future public health strategies to mitigate intracity and cross-border pathogen and ARG transmission.

Coping with shift work-related circadian disruption: A mixed-methods case study on napping and caffeine use in Australian nurses and midwives.

INTRODUCTION: Two of the most ubiquitous fatigue countermeasures used by shift-working nurses are napping and caffeine. This mixed-methods case study investigated the ways nurses and midwives utilised napping and caffeine countermeasures to cope with shift work, and associated sleep, physical health and psychological health outcomes. MATERIALS AND METHODS: N = 130 Australian shift-working nurses and midwives (mean age = 44 years, range = 21-67, 115F, 15M) completed the Standard Shiftwork Index. A sub-set of 22 nurses and midwives completed an in-depth interview. RESULTS: Nearly 70% of participants reported napping. Those who napped during night shifts had significantly less total sleep time before (F2,75 = 5.5, p < 0.01) and between days off (F2,82 = 3.9, p < 0.05). By the end of the night shift, average hours of time awake were significantly less for prophylactic and on-shift nappers compared to non-nappers (F2,85 = 97.2, p < 0.001). Since starting shift work, the percentage of high caffeine consumers (>400 mg/day) increased from 15% to 33% of the sample and an average of 4 (SD = 2) caffeinated beverages per day was reported. Increased caffeine consumption was associated with greater sleep disturbance (r = 0.26, p < 0.05), psychological distress (r = 0.37, p < 0.001), abdomen pain (r = 0.27, p < 0.05) and weight gain since starting shift work (r = 0.25, p < 0.05). Interviews confirmed these relationships and revealed that caffeine consumption on night shift was common, whereas napping on night shift was dependent on a number of factors including ability to sleep during the day. CONCLUSION: This study identified reasons shift workers chose to engage in or abstain from napping and consuming caffeine, and how these strategies related to poor sleep and health outcomes. Further research is required to help develop recommendations for shift workers regarding napping and caffeine consumption as fatigue countermeasures, whilst taking into account the associated hazards of each strategy.

Low frequency dove coos vary across noise gradients in an urbanized environment.

Urbanization poses a challenge to bird communication due to signal masking by ambient noise and reflective surfaces that lead to signal degradation. Bird species (especially oscines) have been shown to alter their singing behaviour to increase signal efficiency in highly urbanized environments. However, few studies on the effects of noise on song structure have included birds with low frequency vocal signals which may be especially vulnerable to noise pollution due to significant frequency overlap of their signals with traffic noise. We compared the perch coos of spotted doves (Streptopelia chinensis), a species with very low frequency vocalizations, in different background noise levels across urban and peri-urban areas in Hong Kong. We documented a 10% upward shift in the minimum frequency of coos of spotted doves across the noise gradient (a relatively small but significant shift), and a reduced maximum frequency in urban habitats with a higher density of built up area. Hong Kong doves had significantly higher minimum and maximum frequencies than doves from throughout their range (from mostly rural sites). Our results indicate that urban species with extremely low sound frequencies such as doves can alter their vocalizations in response to variable urban acoustic environments.

Asymmetric response patterns to subspecies-specific song differences in allopatry and parapatry in the gray-breasted wood-wren.

Song divergence between closely related taxa may play a critical role in the evolutionary processes of speciation and hybridization. We explored song variation between two Ecuadorian subspecies of the gray-breasted wood-wren (Henicorhina leucophrys) and tested the impact of song divergence on response behaviors. Songs were significantly different between the two subspecies, even between two parapatric populations 10 km apart. Playback experiments revealed an asymmetric response pattern to these divergent subspecies specific songs; one subspecies responded more to songs of its own subspecies than to the other subspecies' songs, whereas the second responded equally strongly to songs of both subspecies. While song parameters revealed a mixed pattern of divergence between allopatric and parapatric populations, the majority of spectral characteristics showed increased divergence in parapatry, suggestive of character displacement. This increased song divergence in parapatry appeared to affect behavioral responses to playback as discriminating responses were most prominent in parapatry and against parapatric songs. The clear behavioral impact of subspecies-specific song differences supports a potential role for song as an acoustic barrier to gene flow. The asymmetric nature of the responses suggests that song divergence could affect the direction of gene flow and the position of the subspecies-specific transition.

Stable isotopes as indicators of altitudinal distributions and movements in an Ecuadorean hummingbird community.

Altitudinal migration and dispersal is an important component of the life history of several temperate and tropical birds but remains poorly understood due to the limited success of mark and recapture techniques. Stable isotopes of hydrogen (deltaD) in rainfall, and to a lesser extent, carbon (delta13C) in plants are known to change with altitude and hence may provide the basis of a technique for tracking the altitudinal movements in birds and other wildlife. We investigated the potential for this technique by measuring delta13C, deltaD, and delta15N values in tail feathers of eight species of hummingbirds ( Phaethornis malaris, P. syrmatophorus, P. guy, Adelomyia melanogenys, Coeligena torquata, C. lutetiae, Metallura baroni, M. williami) along an altitudinal gradient (300-3,290 m asl) in the Andes Mountains of Ecuador. Feather delta13C and deltaD values were correlated and each changed significantly with elevation above 400 m. In general, we found good agreement between feather deltaD values and those predicted from a generalized relationship of precipitation and surface water deltaD with altitude. Similarly, feather delta13C values showed an enrichment of approximately 1.5 per thousand per 1,000 m over the linear portion of the elevational response. Stable-nitrogen isotope values were variable, and so did not provide useful information on elevation in birds, apart from trophic effects. Overall there appears to be good potential for using the (deltaD, delta13C) stable isotope approach to track altitudinal movements and to elucidate previously unrecognized patterns of life history variation in both temperate and tropical species that migrate across elevational isotopic gradients.